29 Aprile 2024

Microbiome studies help explore treatments for genetic disorders

The University of Trento has discovered a collection of molecular tools to rewrite DNA through an even more efficient and precise genome editing. Researchers are investigating the clinical development of a group of enzymes for sub-retinal injections. Their work has been published in Nature Communications

Anna Cereseto and Nicola Segata of the Department of Cellular, Computational and Integrative Biology of the University of Trento have joined forces and combined their expertise to develop new therapies for the treatment of genetic diseases. She leads a laboratory that develops advanced genome editing technologies and their application in the medical sector. He is the head of a laboratory of metagenomics, where he studies the variety and characteristics of the human microbiome and its role in health. Their collaboration has led to the identification, in a bacterium of the intestine, of new Crispr-Cas9 molecules that could have a clinical potential to treat genetic diseases such as, for example, retinitis pigmentosa, through sub-retinal injections. 
A study, with Anna Cereseto and Nicola Segata as corresponding and senior authors, has been published in Nature Communications.
Researchers all over the world are investigating genomic therapies to find new treatments for genetic disorders. Genome editing using the Crispr-Cas9 system is based on the use of the Cas9 protein, which works like a pair of molecular scissors that can be programmed to make specific modifications in the genome to cut or replace harmful DNA sequences, correcting the mutations that cause diseases. This biotechnology was discovered in 2012 in the United States and has already led to one approved therapy, a drug for sickle cell disease.
Now the study conducted by the University of Trento brings genomic research one step forward.
"Compared with other Crispr-Cas9 approaches, the one we have identified is precise and effective, and more compact. This new Crispr-Cas9 molecule, as demonstrated by our experiments in the retina, will be more easily delivered to the organs that must be treated in therapies for genetic diseases," says Anna Cereseto, who has been involved in studies on the genomic editor since 2018 with the development of evoCas9.
Expanding the range of Crispr-Cas tools is necessary to speed up the development of therapies for genetic diseases. This can be made by modifying natural enzymes, as was the case with evoCas9, but discovering already evolved enzymes that can work offers great advantages. The collaboration with the laboratory of Computational Metagenomics of Nicola Segata has allowed the laboratory of Molecular Virology of Anna Cereseto to shed light on a vast natural reserve of Crispr-Cas9 systems from which to draw new valuable tools for human genome editing.
"By interrogating a microbiome genome database that we have created over several years, we discovered a large number of Cas9 with interesting properties for genome editing," say Anna Cereseto and Nicola Segata. "We have discovered a great variety of Crispr-Cas9 in the bacteria that inhabit the intestine. In particular, we have identified the CoCas9 nuclease, a very active group of enzymes with a small molecular size, about a thousand amino acids, in Collinsella, a bacterial genus that is often found in human guts."
"The sequencing of the entire microbiome using a metagenomic approach, followed by the laboratory reconstruction of the assembled genomes, has led to the identification of a huge variety of species. The discovery of a collection of new Cas9 nucleases, including CoCas9, makes the genome editing toolkit even larger," they point out.
They conclude: "The difficulty of administration still hampers the development of therapies for genetic diseases. However, CoCas9, thanks to its small size, shows potential for gene therapy applications and is therefore a potential candidate for optimisation through engineering approaches, which deserve further investigation. We are already working on clinical development projects."

About the article

The study on the new "molecular scissors" has been coordinated by Anna Cereseto and Nicola Segata of the University of Trento in collaboration with several Italian and international partners. The article "CoCas9 is a compact nuclease from the human microbiome for efficient and precise genome editing" (doi has been published in Nature Communications and is available in Open Access at: 
Here is the full list of authors: Eleonora Pedrazzoli, Michele Demozzi, Elisabetta Visentin, Matteo Ciciani, Ilaria Bonuzzi, Lorenzo Lucchetta, Giulia Maule, Simone Amistadi, Nicola Segata e Anna Cereseto (Department of Cellular, Computational and Integrative Biology - Cibio, University of Trento); Laura Pezzè and Antonio Casini (Alia Therapeutics, Trento); Annarita Miccio (Université de Paris, Imagine Institute, Laboratory of chromatin and gene regulation); Federica Esposito, Mariangela Lupo e Alberto Auricchio (Telethon Institute of Genetics and Medicine, Pozzuoli).